구강암 세포주에서 실망초 추출물의 항암효능
Anticancer effect of methanol extract of Erigeron bonariensis on oral cancer cells
장분실, 임선아,
소속 상세정보
장분실 ( Jang Boon-Sil ) - Sorabol College Department of Dental Hygiene
임선아 ( Lim Sun-A ) - Songwon University College Department of Dental Hygiene
Abstract
Objectives: Erigeron bonariensis is a type of Erigeron found throughout the tropical and subtropical areas as one of the perennial plants or pioneer plants. It is known to show detoxifying, antipyretic, and anticancer effects for various cancers. However, there are no reports on the anticancer effect of E. bonariensis on oral cancer cells. In the present study, we investigated the effects of the methanol extract of Erigeron bonariensis (MEEB) on the inhibition of cell growth and induction of apoptosis in mucoepidermoid carcinoma (MEC) cell lines, including the MC3 and YD15 oral cancer cells.
Methods: MC3 Cells were treated by dimethyl sulfoxide (DMSO) or methanol extracts of 20 various natural products 20 μg/mL for 48 hours and cell viability were analyzed as Trypan blue exclusion assay. The effects of MEEB treatment on the cell viability of MC3 and YD15 cells, for 48 h, were analyzed by Trypan blue exclusion assay. The anticancer efficacy and apoptosis of oral cancer cell lines were analyzed by western blot analysis. The statistical significance of differences between groups was analyzed by Student’s two-tailed t-test. A value of P<0.05 compared to the vehicle control was considered statistically significant.
Results: Among 20 different naturally derived products, MEEB significantly inhibited cell viability and increased cleaved poly [ADP-ribose] polymerase 1 (PARP) protein in the MC3 and YD15 cells in a concentration-dependent manner.
Conclusions: These results suggest that MEEB can be used as a natural anticancer drug for the treatment of human oral cancer.
키워드
구강암; 단백질; 세포사멸; 실망초; 클리브드-팝
Apoptosis; Cleaved PARP; Erigeron bonariensis; Oral cancer; Protein
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